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Chromatin isolation by RNA purification (ChIRP) in human cardiac tissue (n = 2). qRT-PCR quantification of NPPA in RNA coprecipitated with 2 independent ChIRP probe sets specific for NPPA-AS1 (“Even” and “Odd”). NPPA-AS1 and GAPDH mRNA was quantified as positive and negative controls, respectively. Results are expressed relative to input RNA.
