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. 2019 Oct 16;5(10):eaaw3896. doi: 10.1126/sciadv.aaw3896

Fig. 3. Noncanonical Wnt5a mimetic peptide (Foxy5) conjugated to 2D hydrogel substrates enhances the mechanosensing and osteogenesis of hMSCs.

Fig. 3

(A) Fluorescence micrographs of hMSCs stained for F-actin (red), nuclei (blue), and the mechanosensing marker YAP (green) or the osteogenic marker RUNX2 (green) (B) and ALP (blue in bright-field) (C), cultured on the RGD, Foxy5 + RGD, and Scram + RGD hydrogels. (D) Analysis of the nuclear localization of YAP determined by the nuclear-to-cytoplasmic fluorescence intensity ratio (N/C ratio) (n = 20) and (E) RUNX2 nuclear localization (n = 20) and (F) ALP expression of representative cells cultured on 2D hydrogels in the different experimental groups (n = 9). Scale bars represent 50 μm in the fluorescence micrographs and 200 μm in the bright-field images. Data are shown as the means ± SD. Statistical significance: *P < 0.05, **P < 0.01, and ***P < 0.001 significant difference.