Skip to main content
. 2019 Oct 16;5(10):eaaw3896. doi: 10.1126/sciadv.aaw3896

Fig. 4. Conjugating Wnt5a mimetic peptide (Foxy5) to a 3D porous hydrogel scaffold promotes hMSC osteogenic gene expression and osteogenic matrix synthesis.

Fig. 4

(A) Schematic illustration of the seeding of hMSCs in the Foxy5/Scram + RGD peptide–functionalized 3D hydrogel scaffolds. (B) Quantitative gene expression of osteogenic markers (type I collagen, RUNX2, ALP, and OPN) in hMSCs seeded in porous hydrogels conjugated with RGD peptide alone (RGD), Foxy5 and RGD peptides (Foxy5 + RGD), or Scram and RGD peptides (Scram + RGD) in osteogenic culture. (C) von Kossa staining, Alizarin Red S staining, and immunohistochemistry staining of type I collagen and (D) quantification of the staining intensities after 14 days of osteogenic culture. Scale bars, 50 μm. Data are shown as the means ± SD (n = 9). Statistical significance: * P < 0.05, **P < 0.01, and ***P < 0.001.