(A) Close-up views of RagA/C binding to the RAPTOR subunit of
mTORC1. The CRDs are shown as transparent surfaces. RAPTOR helices contacting
switch I and interswitch of RagA are shown as cylinders. Spheres mark
RAPTOR/RagA interface residues.
(B) View of the interface, illustrating regions with a decrease in
HDX (blue) upon formation of the RagA/C/RAPTOR complex.
(C) Mutational analysis of the binding interface. Strep-tagged
wild-type RAPTOR (WT) and three different RAPTOR mutants (WC(593,594)AA,
RD(597,598)AA and TDH(634-636)AAA) were assayed for their ability to pull-down
RagA-Q66LGTP/RagC-T90NGDP
in vitro. The pull-down efficiencies of RAPTOR mutants were
normalized to WT RAPTOR. Values are means from three independent experiments,
and error bars show standard deviations.