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. Author manuscript; available in PMC: 2020 Mar 9.
Published in final edited form as: Nat Med. 2019 Sep 9;25(9):1402–1407. doi: 10.1038/s41591-019-0568-2

Extended Data Fig. 3. Sorting of NZM-activated memory CD4+ T cells from MS patients.

Extended Data Fig. 3

Flow cytometry analysis of memory CD4+ T cells at day 12 after ex-vivo stimulation with irradiated autologous monocytes untreated (upper panels) or pre-pulsed with NZM peptide pool (lower panels). CFSElowCD25+ICOS+ T cells reactive to NZM peptide pool were FACS-sorted and cloned by limiting dilution (representative of n = 2 biologically independent samples).