a-b, Motif enrichment (a) and ChIP-signals for DREF
and Trl/GAF (b) in hkCP and dCP enhancers (NS: not
significant [FDR-corr. hypergeometric
P>0.01]; boxes: median and
interquartile range; whiskers: 5th and
95th percentiles; two-sided
Wilcoxon-rank-sum P-values).
c, Luciferase assays (LAs) for 4 wildtype and
DRE-motif-mutant hkCP enhancers (numbers: mutated motifs;
error-bars: s.d. [n=3]; * P<0.005
[one-sided t-test]). d, LAs
for 2 dCP enhancers (-) and Trl/GAGA→DRE-mutant
variants (+) with hkCP (top) and dCP (bottom; details as in
c). e, LAs for 6 DRE motifs with hkCP and dCP
(details as in c). f, Model: housekeeping genes
contain Motifs 1,5,6,7 and/or TCT and are activated by
TSS-proximal hkCP enhancers via DREF. Regulated genes
contain TATA-box, Initiator, MTE and/or DPE and are
activated by distal dCP enhancers via Trl/GAF.