Average hkCP (top) and dCP (bottom) S2 STARR-seq
enrichment in 40kb intervals around transcription
starting sites (TSS) that contain different
combinations of known core promoter motifs. Shown
are (left to right) TATA-Initiator (179 TSSs),
Initiator (that do not contain either TATA or DPE;
1901), Initiator-DPE (100), TCT (303) and
Motif1-Motif6 (266). According to their motif
contents, the first 3 are developmental-type core
promoters and the last 2 are housekeeping-type core
promoters. Indeed, only the housekeeping-type core
promoters show a strong enrichment of hkCP S2
STARR-seq signals at the TSS, which is not seen for
the dCP STARR-seq signal (due to
enhancer–core promoter specificity) nor for
the developmental-type core promoters (due to the
dCP enhancers location at more distal sites).