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. Author manuscript; available in PMC: 2020 Mar 4.
Published in final edited form as: Nature. 2019 Sep 4;573(7773):287–290. doi: 10.1038/s41586-019-1530-7

Extended Data Fig. 8. Effect of Nb8205 on FluPolA activity and mapping of host adaptive mutations at the FluPolA dimer interface.

Extended Data Fig. 8

a, b, Effect of Nb8205 on in vitro ApG-primer replication by FluPolA on a vRNA (a) and cRNA (b) template. Data are mean ± s.e.m., n=3 independent reactions. c, Omitting UTP from in vitro ApG-primer replication by FluPolA on a cRNA template affects the synthesis of the 15 nucleotide full-length vRNA but not of the 12 nucleotide short vRNA. The position in the template at which UTP is required is indicated in red. Representative data from n=2 independent reactions. For gel source data, see Supplementary Fig. 2. d, Crystal structure of H3N2 FluPolA with amino acid residues implicated in avian to mammalian host adaptation of influenza A viruses indicated52–66.