Extended Table 2. Cryo-EM data collection, refinement and validation statistics.
| FluPolA-cRNA | FluPolA-cRNA-Nb8205 | FluPolB-cRNA | Flu PolA-vRNA | |||
|---|---|---|---|---|---|---|
| Dimer | Monomer | Dimer | Monomer | Monomer | ||
| EMD-4664 | EMD-4663 | EMD-4661 | EMD-4666 | EMD-4660 | EMD-4986 | |
| PDB 6QX8 | PDB 6QX3 | PDB 6QXE | PDB 6QWL | PDB 6RR7 | ||
| Data collection and processing | ||||||
| Magnification | 130,000 | 130,000 | 130,000 | 130,000 | ||
| Voltage (kV) | 300 | 300 | 300 | 300 | ||
| Electron exposure (e–/Å2) | 32.92 | 30.00 | 31.10 | 36.60 | ||
| Defocus range (μm) | -0.5 to -0.7 | -0.5 to -0.7 | -1.3 to -2.5 | -0.5 | ||
| Pixel size (Å) | 1.043 | 1.080 | 1.080 | 1.085 | ||
| Symmetry imposed | Cl | C2 | Cl | Cl | Cl | Cl |
| Initial particle images (no.) | 56,070 | 505,860 | 406,945 | 1,012,085 | 2,210,168 | |
| Final particle images (no.) | 36,913 | 52,932 | 27,861 | 41,549 | 170,144 | |
| Map resolution (Å) | 4.34 | 4.07 | 3.79 | 4.15 | 4.18 | 3.01 |
| (FSC threshold 0.143) | ||||||
| Map resolution range (Å) | 3.97-7.82 | 3.84-6.35 | 3.70-5.41 | 3.85-7.43 | 3.90-6.30 | 2.82-5.03 |
| 3DFSC spherity* | 0.758 | 0.787 | 0.851 | 0.772 | 0.781 | 0.909 |
| CryoEF score** | 0.41 | 0.53 | 0.72 | 0.50 | 0.68 | 0.50 |
| Refinement | ||||||
| Initial model used (PDB code) | - | 6QNW | 6QPG | 6QX8/6QPG | 5EPI | 6QNW |
| Model resolution (Å) | 4.34 | 4.07 | 3.79 | 4.15 | 4.18 | 3.01 |
| (FSC threshold 0.143) | ||||||
| Model resolution range (Å) | 3.97-7.82 | 3.84-6.35 | 3.70-5.41 | 3.85-7.43 | 3.90-6.30 | 2.82-5.03 |
| Map sharpening B factor (Å2) | -80 | -100 | -110 | -95 | -57 | -65 |
| Model composition | ||||||
| Non-hydrogen atoms | - | 38,596 | 21,589 | - | 10,409 | 36,306 |
| Protein residues | - | 2,384 | 1,337 | - | 1,244 | 2,228 |
| Nucleotide (RNA) | - | 24 | 14 | - | 26 | 30 |
| B factors (Å2) | ||||||
| Protein | - | 68.60 | 71.24 | - | 151.24 | 22.39 |
| Nucleotide (RNA) | - | 64.94 | 86.46 | - | 207.12 | 24.68 |
| R.m.s. deviations | ||||||
| Bond lengths (Å) | - | 0.007 | 0.004 | - | 0.008 | 0.007 |
| Bond angles (°) | - | 1.158 | 0.750 | - | 1.006 | 0.820 |
| Validation | ||||||
| MolProbity score | - | 1.85 | 1.66 | - | 2.1 0 | 1.31 |
| Clashscore | - | 6.48 | 3.24 | - | 10.07 | 1.90 |
| Poor rotamers (%) | - | 0.14 | 0 | - | 0.93 | 0 |
| Ramachandran plot | ||||||
| Favored (%) | - | 91.86 | 90.34 | - | 89.07 | 94.88 |
| Allowed (%) | - | 7.97 | 9.66 | - | 10.85 | 5.12 |
| Disallowed (%) | - | 0.17 | 0 | - | 0.08 | 0 |