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. 2019 Oct 15;6(5):ENEURO.0172-19.2019. doi: 10.1523/ENEURO.0172-19.2019

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Copyright © 2019 Akhter et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 7. — KCC2 depletion occurs independent of microgliosis. A, Relative KCC2 depletion in WT and CX3CR1 BDNF KO animals 14 d after cut/ligation. Genotype had no effect on KCC2 levels in motoneurons contralateral or ipsilateral to injury (Table 5). Removing BDNF from microglia had no impact on KCC2 expression. B, Lumbar spinal cord sections from animals expressing normal CSF1 (top) and with CSF1 removed from motoneurons (bottom) 14 d after ligation. Chat^IREScre/+: : csf1^f/f animals (bottom image) exhibit normal microgliosis in the dorsal horn but have the microglial reaction to injury greatly attenuated in the ventral horn compared to Chat^I+/+: : csf1^f/f(top image). Scale bars = 200 μm. C, KCC2 immunofluorescence 14 d after sciatic ligation in csf1^f/f animals. Preventing microgliosis in the ventral horn had no impact on KCC2 within intact or injured motoneurons (Table 5). Error bars = SEM; **p ≤ 0.01, ***p ≤ 0.001.