Table 2.
Primary antibodies used for immunohistochemistry (IHC) and Western blottings (WB)
| Antigen | Immunogen | Host/type | Manufacturer | RRID # | dilution | Use |
|---|---|---|---|---|---|---|
| KCC2 | N-terminal His-tag fusion protein; pan-rat KCC2; aa 932–1043 | Rabbit/ polyclonal | Millipore catalog #07-432, | AB_11213615 | 1:500 | IHC |
| NeuN | Purified cell nuclei from mouse brain | Mouse/ monoclonal A60 clone | EMD Millipore catalog #MAB377 | AB_2298772 | 1:500 | IHC |
| EGFP | Recombinant GFP 6-his tag | Chicken/ polyclonal | Serotec catalog #obt1644 | AB_10000240 | 1:1000 | IHC |
| Iba1 | C terminus of Iba1 | Goat/ polyclonal | Novus catalog #NB 100-1028, | AB_521594 | 1:500 | IHC |
| mCherry | Purified recombinant peptide produced in E. coli | Goat/ polyclonal | MyBioSource Catalog #MBS448050, | NA | 1:100 | IHC |
| NF-H | Purified NF-H from bovine brain | Chicken/ polyclonal | Aves catalog #NFH | NA | 1:500 | IHC |
| VAChT | Recombinant protein; rat VAChT; aa 475–530 | Guinea pig/ polyclonal | Synaptic Systems catalog #139105 | NA | 1:500 | IHC |
| ChAT | Human placental enzyme | Goat/ polyclonal | Millipore catalog #AB144P | AB_2079751 | 1:100 | IHC |
| ATF3 | Recombinant protein corresponding to aa MMLQHPGQVSASEVSASAIVPCLSPPGSLVFEDFANLTPFVKEELRFAINQNKHLCHRMSSALESVTVSDRPLGVSITKAIVAPEEDERKKRRRERNKIAAAKCRNKKKEKTEC | Mouse/ monoclonal | Novus NBP2-34489 | NA | 1:200 | IHC |
| Phospho-p44/42 MAPK (Erk1/2) | Synthetic phosphopeptide; human p44 MAP kinase; Thr202/Tyr204 residues | Rabbit/ polyclonal | Cell Signaling Technology catalog #9101 | AB_331646 | 1:1000 | WB |
| β-actin | Synthetic peptide; human β-actin; N-terminal region | Rabbit/ polyclonal | Novus catalog #NB600-503 | NA | 1:5000 | WB |