Table 1:
Primary and secondary antibodies used in the study
| Anti | Host | Isotype | Dilution | Company (catalog #) |
|---|---|---|---|---|
| Primary antibodies | ||||
| AC3 | Rabbit | Polyclonal IgG | 1:300 | Thermo Fisher Scientific (PA5-35382) |
| BrdU | Rat | Monoclonal IgG | 1:300 | Abcam (ab6326) |
| BrdU | Rat | Monoclonal IgG | 1:300 | Accurate Chemical (OBT 0030) |
| cCasp3 | Rabbit | Polyclonal IgG | 1:500 | Cell Signaling Technology (9661) |
| GAP 43 | Rabbit | Polyclonal IgG | 1:1000 | Millipore-Chemicon (AB5220) |
| Ki67 | Rabbit | Polyclonal IgG | 1:200 | Novus (110-89719) |
| OMP | Goat | Polyclonal IgG | 1:1000 | Wako (544-10001) |
| ZsGreen | Rabbit | Polyclonal IgG | 1:300 | Clontech (632474) |
| Anti | Host | Label | Dilution | Company |
| Secondary antibodies | ||||
| Goat | Donkey | A555 | 1:1000 | Thermo Fisher Scientific |
| Goat | Donkey | A647 | 1:1000 | Thermo Fisher Scientific |
| Rat | Donkey | A488 | 1:1000 | Thermo Fisher Scientific |
| Rabbit | Donkey | A488 | 1:1000 | Thermo Fisher Scientific |
| Rabbit | Donkey | A555 | 1:1000 | Thermo Fisher Scientific |