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. 2019 Oct 10;13:458. doi: 10.3389/fncel.2019.00458

FIGURE 3.

FIGURE 3

Altered excitatory and inhibitory spontaneous synaptic transmissions in global Shank3Δ14–16, but not Emx1-Cre;Shank3Δ14–16, mPFC layer 2/3 pyramidal neurons. (A,B) Global Shank3Δ14–16 mice (P21–26) show increased frequency, but normal amplitude, of mEPSCs and normal mIPSCs in layer 2/3 pyramidal neurons in the prelimbic region of the mPFC. Data are shown as mean ± SEM. n = 17 neurons from 5 mice (WT), 17, 7 (KO) for mEPSCs, 15, 5 (WT), 16, 6 (KO) for mIPSCs, ∗∗P < 0.01, ns, not significant, Student’s t-test. (C,D) Emx1-Cre;Shank3Δ14–16 mice (P18–22) show normal mEPSCs and mIPSCs in layer 2/3 pyramidal neurons in the prelimbic region of the mPFC. n = 19, 4 (WT), 16, 4 (cKO) for mEPSCs, 14, 4 (WT), 17, 4 (cKO) for mIPSCs, Student’s t-test (amplitude of mEPSC and frequency of mIPSC), and Mann–Whitney U test (frequency of mEPSC and amplitude of mIPSC). (E,F) Global Shank3Δ14–16 mice (P21–24) show normal sEPSCs and increased frequency, but normal amplitude, of sIPSCs in layer 2/3 pyramidal neurons in the prelimbic region of the mPFC. Data are shown as mean ± SEM. n = 17 neurons from 3 mice (WT), 17, 3 (KO) for sEPSCs, 15, 3 (WT), 18, 3 (KO) for sIPSCs, ∗∗P < 0.01, ns, not significant, Student’s t-test. (G,H) Emx1-Cre;Shank3Δ14–16 mice (P20–25) show normal sEPSCs and sIPSCs in layer 2/3 pyramidal neurons in the prelimbic region of the mPFC. n = 15, 3 (WT), 16, 3 (cKO) for sEPSCs,19, 3 (WT), 19, 3 (cKO) for sIPSCs, ns, not significant, Student’s t-test (frequency and amplitude of sEPSC and frequency of sIPSC), and Mann–Whitney U test (amplitude of sIPSC).