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. 2019 Oct 10;13:458. doi: 10.3389/fncel.2019.00458

FIGURE 6.

FIGURE 6

Normal locomotor activity in open-field and Laboras cages, and enhanced anxiety-like behavior in elevated plus-maze (EPM) in Emx1-Cre;Shank3Δ14–16 mice. (A,B) Emx1-Cre;Shank3Δ14–16 mice (A, 11–17 weeks; B, 10–17 weeks) show normal levels of locomotor activity in the open-field test (A) and in Laboras cages (B). Note that these mice spend a normal amount of time in the center region of the open-field arena, indicative of normal anxiety-like behavior in this test. Data are shown as means ± SEM; data values in the bar graph in panel B represent those from light-off periods (shaded durations). n = 23 (WT), 21 (cKO) for open-field and 13 (WT), 14 (cKO) for Laboras, repeated measures of two-way ANOVA (for the left panels in open-field and Laboras; genotype p-values = 0.6889 and 0.3025, respectively), Student’s t-test (for the right panels in open-field and first 6 h of the right panels in Laboras), and Mann–Whitney U test (for the last 72 h of the right panels in Laboras). (C) Emx1-Cre;Shank3Δ14–16 mice (14–19 weeks) spend an increased amount of time in the open arm of the elevated plus-maze (EPM). n = 23 (WT), 20 (cKO), ∗P < 0.05, ∗∗∗P < 0.001, Student’s t-test (right panels of elevated plus maze test) and paired t-test (for left panels of elevated plus maze test). (D) Emx1-Cre;Shank3Δ14–16 mice (14–19 weeks) spend a normal amount of time in the light chamber of the light-dark (LD) apparatus. n = 23 (WT), 21 (cKO), ns, not significant, Student’s t-test.