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. 2019 Oct 10;10:1273. doi: 10.3389/fpls.2019.01273

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Copyright © 2019 Santoni, Ciardiello, Zampieri, Pezzotti, Giangrieco, Rafaiani, Ciancamerla, Mari and Avesani

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Time-course analysis of pK7WG2. Betv1 expression of the agro-infiltrated N. benthamiana leaves. Western blot analysis (A) and corresponding loading controls (RuBisCO large subunit) stained with Coomassie Brilliant Blue (B), of pBet v 1a containing protein extract from leaves samples collected from 3 to 14 dpi. Each lane was loaded with 2.5 µg of TSP, the western blot was probed with anti-FLAG® antibody conjugated with horseradish peroxidase. Side numbers indicate molecular mass markers in kDa. p.c., positive control, 10 ng of a commercially available flagged protein; n.c., negative control, extract from leaves infiltrated solely with A. tumefaciens EHA105.