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. 2019 Oct 10;10:1273. doi: 10.3389/fpls.2019.01273

Figure 2.

Figure 2

Evaluation of extraction reproducibilty of the pG PVX GATEWAY(A).Betv1 agro-infiltrated N. benthamiana leaves. Western blot analysis (A) and corresponding loading controls (RuBisCO large subunit) stained with Coomassie Brilliant Blue (B), of pBet v 1a containing protein extract from leaves samples in three biological replicates. Each lane was loaded with 2.5 µg of TSP, the western blot was probed with anti-FLAG® antibody conjugated with horseradish peroxidase. Side numbers indicate molecular mass markers in kDa. p.c., positive control, 10 ng of acommercially available flagged protein; n.c., negative control, extract from leaves infiltrated solely with A. tumefaciens GV3101.