Figure 3.

Evaluation of the pBet v 1a Purification, from pK7WG2. Betv1 agroinfected leaves, using a imidazole gradient. Western blot analysis (A) and corresponding Silver Staining (B), of 1.5 mL purification elutions. Each lane was loadedwith 5 µL of elution, the western blot was probed with anti-FLAG® antibody conjugated with horseradish peroxidase. Side numbers indicate molecular mass markers in kDa. p.c., positive control, 10 ng of a commercially available flagged protein; n.c., negative control, from leaves infiltrated solely with A. tumefaciens EHA105, submitted to the same purification protocol as the leaves extract containing pBet v 1a. The bottom bar of the B image stands for the imidazole concentration for every gradient fraction.