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. 2019 Oct 10;10:2366. doi: 10.3389/fimmu.2019.02366

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Copyright © 2019 Mangogna, Belmonte, Agostinis, Zacchi, Iacopino, Martorana, Rodolico, Bonazza, Zanconati, Kishore and Bulla.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative immunohistochemical analysis of C3d and C4d complement activation products in low grade (A,D) and high grade (B,E) gliomas. C3d and C4d specific staining is segregated in the blood vessels, while the tumor tissues are negative. Liver staining (C,F) represents the antigen (tissue) control. Polymer detection system with AEC (red) chromogen; scale bars, 50 μm.