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. 2019 Oct 10;10:1242. doi: 10.3389/fpls.2019.01242

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Copyright © 2019 An, Li, Yang, Zhang, Zhao, Wu, Wang and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The interactions of TaFBA1 with RCAR1 (an ABA receptor) and ABI5. (A) Interactions of TaFBA1 with RCAR1 and ABI5 were analyzed via the yeast two-hybrid assay. Interaction between pGADT7-324 and pGBKT7-332, pGADT7-322 and pGBKT7-332, were used as a positive and negative controls, respectively. LT, LYH, and LTHA represent the selective media SD-Leu-Trp, SD-Leu-Trp-His, and SD-Leu-Trp-His-Ade, respectively. (B) BiFC assays were used to determine the interaction between TaFBA1 and RCAR1 using yellow fluorescence signal. nYFP denotes expression of the YFP N-terminal fusion construct. cYFP denotes expression of the YFP C-terminal fusion construct.