TABLE 1.

Cultivation conditions and stress treatments summary.

Condition		Time of harvest (h)	OMV quantification	OMV isolation for TEM	SEM of bacteria	Proteomic comparison
Untreateda	37°C, pH 6.8	16	Yes	Yes	Yes	Yes
High temperature	42°C	16	Yes	Yes	Yes	Yes
Low temperature	25°C	16/40	Yes/yes	Yes/no	Yes/no	Yes/no
pH	7.4	16	Yes	No	No	No
	6.3	16	Yes	No	No	No
	5.8	16	Yes	No	No	No
	5.3	16/40b	Yes/yes	Yes/no	Yes/no	Yes/no
	4.8	16	No	No	No	No
	4.3	16	No	No	No	No
Oxidative stress	5 mM H2O2	12 h of standard cultivation, 4 h of treatment	Yes	No	No	No
	10mM H2O2		Yes	Yes	Yes	Yes
	50mM H2O2		Noc	No	No	No
Agar plate	McLeod	24	No	Yes	Yes	Yes

^aUntreated control, FSC200 cultivated in BHI pH 6.8, at 37°C for 16 h (late logarithmic phase); represents 100% of the vesiculation rate. ^bAt pH 5.3, the growth is delayed but it reaches untreated level at ca. 40 h of cultivation (see growth curves in Figure 4C). ^c50 mM H₂O₂ had a considerable bactericidal effect (Figure 4A), large amount of dead cells distorted OMV quantification.