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. 2019 Oct 10;10:1278. doi: 10.3389/fphys.2019.01278

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Copyright © 2019 Rathje, Randle, Al Rawi, Skinner, Nelson, Majumdar, Johnson, Bacon, Vlazaki, Affara, Ellis and Laman.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Male mice with reduced expression of Fbxo7 are sterile. (A) Colony data showing the genotypes of animals (n = 481) born from matings between heterozygous parents. All three potential genotypes are observed in the offspring, but the proportion of homozygous Fbxo7LacZ/LacZ offspring is lower than expected (^∗∗∗ chi-squared goodness-of fit test p = 0.007 vs. Mendelian 1:2:1 expectation). (B) Average testis weight for each genotype (n = 6; WT and homozygous; n = 4, heterozygous LacZ; One-way ANOVA, ^∗∗∗p = 0.001067). (C) Wild type cauda epididymis showing large numbers of stored sperm. (D) Fbxo7LacZ/LacZ cauda epididymis showing very few degenerating sperm. (E,F) High resolution zoom of sections (C,D). Dotted line indicates the border of the tubule lumen in each view. (G) Montage of DAPI-stained sperm nuclei showing the spectrum of sperm morphologies present (see also Supplementary Figure S1). (H) FACS quantitation of testis cells according to DNA content as measured by propidium iodide staining (note that highly condensed spermatids and mature sperm were not quantitated); n = 4 WT, 2 heterozygous, 2 homozygous LacZ mice.