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. 2019 Oct 16;9:14880. doi: 10.1038/s41598-019-51369-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Root colonisation by arbuscular mycorrhizal fungal structures (a), and normalised expression of the AMF biomass marker gene α-tubulin (b) in the roots of Medicago truncatula plants inoculated with the AMF R. irregularis, and grown at different soil Zn (Zn0-Zn20) and P (P0-P50) concentrations. Values are mean ± SEM, n = 5. Means labelled with the same letter were not significantly different at the P < 0.05 level (Tukey’s HSD), see Table 2 for details of ANOVA results.