Table 3.
Quantitative PCR Primer Pairs for the Quantification of Genes of Interest.
| Target Gene | Primer | Sequence | Size of Target | Tm | R2 | Amplification efficiency (%) | Reference |
|---|---|---|---|---|---|---|---|
| 16s | 338 F | ACTCCTACGGGAGGCAGCAG | 197 | 62 | 0.99 | 102 | 52 |
| 518 R | ATTACCGCGGCTGCTGG | ||||||
| E. coli (UidA) | Uida 405 F | CAACGAACTGAACTGGCAGA | 121 | 60 | 0.97 | 108 | 53 |
| Uida 405 R | CATTACGCTGCGATGGAT | ||||||
| Pseudomonas spp. | Pse435F | ACTTTAAGTTGGGAGGAAGGG | 251 | 62 | 0.99 | 94 | 54 |
| Pse686R | ACACAGGAAATTCCACCACCC | ||||||
| Enterococcus (Ent) | Ent376F | GGACGMAAGTCTGACCGA | 220 | 62 | 0.99 | 92 | 55 |
| Ent578R | TTAAGAAACCGCCTGCGC | ||||||
| Sul 1 | SulI-F | CGCACCGGAAACATCGCTGCAC | 163 | 65 | 0.99 | 98 | 56 |
| SulI-R | TGAAGTTCCGCCGCAAGGCTCG | ||||||
| Sul 2 | SulII-F | CTCAATGATATTCGCGGTTTYCC | 245 | 62 | 0.96 | 93 | 57 |
| SulII-R | AAAAACCCCATGCCGGGRTC | ||||||
| Sul 3 | sul3-F | GAGCAAGATTTTTGGAATCG | 128 | 60 | 0.99 | 109 | 56 |
| sul3-R | CTAACCTAGGGCTTTGGA | ||||||
| Tet (B) | tetB F | TACGTGAATTTATTGCTTCGG | 206 | 60 | 0.97 | 96 | 58 |
| tetB R | ATACAGCATCCAAAGCGCAC | ||||||
| BlaOXA-48 | BlaOXA F | GCGTGGTTAAGGATGAACAC | 438 | 61 | 0.99 | 98 | 59 |
| BlaOXA R | CATCAAGTTCAACCCAACCG | ||||||
| BlaCTX-M | blaCTX-M F | ATTCCRGGCGAYCCGCGTGATACC | 227 | 65 | 0.99 | 92 | 60 |
| blaCTX-M R | ACCGCGATATCGTTGGTGGTGCCAT |