Table 1.
Histopathological analysis, ZN stain, ELISA, PCR and bacteriological culture results from all the animals included in the current study.
| ID | Histopathological analysis | ELISA (OD) | Fecal PCR | Fecal culture (CFUs) | Gut tissues | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Microscopic | Macroscopic | ZN | Pre-mortem | At slaughter | Pre-mortem | At slaughter | Pre-mortem | At slaughter | PCR | Culture | |
| 1 | Negative | No | Neg | Neg (1.26) | Neg (1.93) | Neg | Neg | Neg | Neg | Neg | Neg |
| 2 | Negative | No | Neg | Neg (2.77) | Neg (8.54) | Neg | Neg | Neg | Neg | Neg | Neg |
| 3 | Negative | No | Neg | Neg (8.99) | Neg (2.45) | Neg | Neg | Neg | Neg | Neg | Neg |
| 4 | Focal | No | Neg | ND | Neg (2.71) | ND | Neg | ND | Neg | Pos (0.38) | Neg |
| 5 | Focal | No | Neg | Neg (1.00) | Neg (1.03) | Neg | Neg | Neg | Neg | Pos (7.50) | Low |
| 6 | Focal | No | Neg | Neg (4.39) | Neg (5.51) | Neg | Neg | Neg | Neg | Pos (305.28) | Neg |
| 7 | Focal | No | Neg | Neg (4.92) | Neg (0.74) | Pos (0.03) | Neg | Neg | Neg | Pos (12.26) | Neg |
| 8 | Focal | No | Neg | Pos (95.85) | Pos (133.17) | Neg | Pos | Neg | Neg | Pos (70.54) | Medium |
| 9 | Multifocal | Yes | Pos | Neg (0.00) | Neg (2.97) | Neg | Neg | Neg | Neg | Neg | Neg |
| 10 | Diffuse paucibacillary | Yes | Pos | Neg (53.00) | Pos (283.13) | Neg | Pos | Neg | Neg | Pos (667.65) | Heavy |
| 11 | Diffuse intermediate | Yes | Pos | Pos (142.31) | Pos (187.69) | Pos (0.14) | Pos (0.14) | Neg | Neg | Pos (3896.19) | Heavy |
| 12 | Diffuse intermediate | Yes | Pos | Pos (174.01) | Pos (241.18) | Pos (7.50) | Pos (114.00) | Neg | Heavy | Pos (104032) | Heavy |
| 13 | Diffuse intermediate | Yes | Pos | Neg (32.19) | Pos (288.75) | Pos (1.41) | Pos (39.88) | Neg | Neg | Pos (1572.24) | Low |
| 14 | Diffuse multibacillary | Yes | Pos | Pos (286.51) | Pos (255.55) | Pos (11.70) | Pos (2832.00) | Neg | Neg | Pos (173316.00) | Heavy |
Neg, Negative; Pos, Positive; ZN, Ziehl-Neelsen; OD, Optical Density; CFU, Colony Forming Units; ND, No Determined. DNA samples with a PCR-positive result using the LSI VetMax Triplex real-time PCR were quantified using the ParaTB Kuanti-VK kit. qPCR results are expressed as MAP DNA copies per gram of feces or tissues × 102.