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. 2019 Oct 16;9:14841. doi: 10.1038/s41598-019-51181-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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De novo transcriptome profiling of germline populations. (a) Venn diagram showing the number of transcripts identified via de novo transcriptome analysis of the four populations. The number of novel transcripts (not yet annotated in the AgamP4.8 genome) is given between parentheses. (b) False-colour k-means clustering heatmap showing the expression as Z-score of log2(FPKM + 1) of non-annotated (novel) transcripts and (c) annotated transcripts that are enriched in each of the four populations (fold change > 2 compared to each of the other three populations and adjusted P-value < 0.05). Only genes that showed expression above 1 FPKM in at least one of the cell populations were included in the de novo transcriptome analysis.