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. 2012 Jan 25;32(4):1467–1480. doi: 10.1523/JNEUROSCI.2272-11.2012

Figure 2.

Figure 2.

Aromatic residues in sorLA are important for the association with retromer proteins. A, Amino acid sequences of human WT and mutant sorLA cytoplasmic tails. Overlapping 15-mer peptides used for pull-down studies are indicated. B, Precipitated proteins are visualized by SDS-PAGE and Coomassie staining. The arrowhead marks the VPS26 subunit identified by MS in several independent experiments. C, Co-IP analysis of CD8 chimeric proteins from transfected HeLa cells. Precipitated proteins were analyzed by WB. The asterisk in lane CD8ED–APPCD–WT indicates an unknown protein unrelated to VPS26. D, Co-IP of VPS35 with sorLA–WT from stably transfected SH-SY5Y cells. E, F, Surface plasmon resonance analysis using 50 nm VPS26 protein or 500 nm GGA1–VHS domain applied to biosensor surfaces immobilized with sorLA–WT and sorLA–FANSHY→6A tails fused to H6Ubi.