Table 1.
MS analysis of anti-CD8 native IP assays from HeLa cell lysates using CD8 reporter proteins
| Protein detected | CD8ED–CIMPRCD–WT | CD8ED–sorLACD–WT | CD8ED–sorLACD–FANSHY→6A |
|---|---|---|---|
| β1-Adaptin | 714 (14) | 0 | 0 |
| γ-Adaptin | 434 (9) | 30 (1) | 0 |
| μ1-Adaptin | 330 (7) | 0 | 0 |
| GGA2 | 0 | 46 (1) | 0 |
| VPS35 | 79 (5) | 426 (7) | 27 (1) |
| VPS26A | 0 | 26 (1) | 0 |
| VPS29 | 0 | 39 (1) | 0 |
| SNX2 | 51 (2) | 0 | 0 |
| SNX6 | 150 (1) | 0 | 0 |
| Rab7A | 36 (1) | 22 (1) | 26 (1) |
| CD8α | 1783 (6) | 1425 (5) | 188 (4) |
| GAPDH | 444 (6) | 93 (4) | 407 (4) |
| EF1A1 | 579 (6) | 394 (5) | 353 (5) |
| TfnR | 424 (12) | 1982 (13) | 184 (8) |
Immunoprecipitates of cells stably expressing the indicated CD8-reporter construct using an antibody against CD8. Values indicate the Mascot score and numbers in parentheses the quantity of peptides detected. The data are based on searching the MaxQuant database.