Table 3.
Comparison of the diagnostic test results obtained by parasite microscopy and molecular (ITS-1 PCR) methods
| PCR | Total | T. spp. | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Tb | Tv | Tc | Tth | Tc + Tv | Tb + Tv | Tth + Tv | Tc + Tb + Tv | Negatives | overlap (%) | ||
| Tb | 1 | 1 | 0 | 6 | 0 | 0 | 1 | 0 | 7 | 16 | 43.8 |
| Tv | 0 | 3 | 0 | 3 | 0 | 2 | 0 | 0 | 1 | 9 | 88.9 |
| Tc | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 3 | 6 | 50.0 |
| Tc + Tv | 1 | 1 | 1 | 0 | 0 | 0 | 3 | 0 | 0 | 6 | 100.0 |
| Tc + Tb | 1 | 3 | 1 | 2 | 0 | 0 | 1 | 0 | 3 | 11 | 72.7 |
| Tc + Tb + Tv | 0 | 1 | 1 | 1 | 0 | 0 | 2 | 0 | 1 | 6 | 83.3 |
| Tv + Tb | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 100.0 |
| Negatives | 47 | 72 | 8 | 206 | 3 | 14 | 64 | 3 | 468 | 885 | 47.1 |
| Total | 50 | 81 | 13 | 219 | 3 | 17 | 71 | 3 | 483 | 940 | 48.6 |
Tb: T. brucei, Tc: T. congolense, Tth: T. theileri / T. grayi, Tv: T. vivax. T. spp: T. all species Animals from Mayo Tsanaga region were not considered because microscopy was not carried out at this location. Only the animals with parasitological and molecular data were considered