Fig. 2.

The paternally methylated secondary DMRs associated with H19 and Cdkn1c display high levels of hemimethylation. Bisulfite mutagenesis and sequencing of F₁ hybrid DNA derived from 7.5 dpc BxC embryos and 5 dpp BxC liver. Individual circles in each row represent one of the potentially methylated CpG dinucleotides analyzed at the H19-ppDMR (a) or the Cdkn1c DMR (b), and each paired row of circles represents the complementary strands of an individual subclone; semi-circles to the right or left indicate the location of the linker connecting the complementary strands. Filled circles represent methylated cytosines, open circles represent unmethylated cytosines, absent circles represent ambiguous data. Alphanumeric labels identify subclones analyzed; letters represent independent amplification reactions, while numbers represent individual subclones. Subclones derived from the same amplification that have identical sequence and methylation patterns are grouped together, as it was not possible to determine if these amplicons were derived from the same or different template molecules. Data obtained from 14.5 dpc BxC embryos and adult BxC liver are shown in Additional file 7: Figure S1. Reciprocal cross-data obtained from 13.5 dpc CxB embryos are shown in Additional file 10: Figure S4