Fig. 4.

Inhibition of EZH2 results in an increased expression of two PRC2 target genes. a, c Relative expression level of the PRC2 target genes, DAG1 (a) and WRKY70 (b), and of the non-target gene SAUR14 (c), in wild-type (Ws-4) seedlings directly grown for 5 days in the presence of increasing concentrations (30, 60, 120 μM) of RDS 3434 or DMSO as control. Relative expression levels were normalized with that of the GAPDHa (At3g26650) gene, and are presented by the ratio of the corresponding mRNA level in the control, which was set to 1. d, e Chromatin from samples derived from 5-days old seedlings grown in the presence of 120 μM RDS 3434 or DMSO as control, immunoprecipitated with anti-H3K27me3 antibodies, or without antibodies as a negative control. The amount of DNA for DAG1 (d) or WRKY70 (e) was measured by qPCR. The values of fold enrichment were normalized to input. All the primers used are listed in Table 1. The results were obtained from two independent replicates with SD values. Significant differences were analyzed by t-test (*P ≤ 0.05, **P ≤ 0.01)