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. 2019 Oct 17;20:59. doi: 10.1186/s40360-019-0340-8

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Knockdown of HIF-1α attenuated Ang II-induced inflammatory cytokine production in podocytes. a Podocytes were transfected with siRNA 934, 1067 or 1217 to knock down HIF-1α expression. Untreated and scramble siRNA transfected podocytes (scramble negative control, SNC) were designated as the normal group. β-Tubulin was used as an equal loading marker. (*p < 0.05 in one-way ANOVA, n = 3). b Podocytes were transfected with siRNA 934 and then stimulated with 10^-7 M Ang II for 12 h. β-Tubulin was used as an equal loading marker. (*p < 0.05 in one-way ANOVA, n = 3). c Podocytes were transfected with siRNA 934 and then stimulated with 10^-7 M Ang II for 12 h. Representative Western blots of MCP-1 and TNF-α expression in the different groups. β-Tubulin was used as an equal loading marker. (*p < 0.05 in one-way ANOVA, n = 3)