Table 1.
Filamentous fungal model strains and cell factories for which CRISPR gene editing tools have been established.
Species | CRISPR protein | Nuclease delivery | References |
---|---|---|---|
Aspergillus fumigatus | Cas9 | Plasmid-based and RNP-based | [36, 37] |
A. nidulans | Cas9, Cpf1 | Plasmid-based | [9] |
A. niger | Cas9, Cpf1 | Plasmid-based and RNP-based | [5, 9] |
A. oryzae | Cas9 | Plasmid-based | [38] |
Neurospora crassa | Cas9 | Plasmid-based | [39] |
Penicillium chrysogenum | Cas9 | Plasmid- based and RNP-based | [33] |
Thermothelomyces thermophilus | Cas9 | Plasmid-based | [12] |
Trichoderma reesei | Cas9 | Plasmid-based | [40] |
Note that the nuclease can be delivered to the cell either plasmid encoded or as a purified protein, which has to be preassembled with in vitro transcribed gRNA to form ribonucleoproteins (RNPs)