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. 2019 Oct 17;6:15. doi: 10.1186/s40694-019-0079-4

Table 1.

Filamentous fungal model strains and cell factories for which CRISPR gene editing tools have been established.

For details, see reviews [68]

Species CRISPR protein Nuclease delivery References
Aspergillus fumigatus Cas9 Plasmid-based and RNP-based [36, 37]
A. nidulans Cas9, Cpf1 Plasmid-based [9]
A. niger Cas9, Cpf1 Plasmid-based and RNP-based [5, 9]
A. oryzae Cas9 Plasmid-based [38]
Neurospora crassa Cas9 Plasmid-based [39]
Penicillium chrysogenum Cas9 Plasmid- based and RNP-based [33]
Thermothelomyces thermophilus Cas9 Plasmid-based [12]
Trichoderma reesei Cas9 Plasmid-based [40]

Note that the nuclease can be delivered to the cell either plasmid encoded or as a purified protein, which has to be preassembled with in vitro transcribed gRNA to form ribonucleoproteins (RNPs)

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