Table 4.
Editing efficiency for four different gene loci of FnCpf1
| Target | RNP | Plasmid | ||||||
|---|---|---|---|---|---|---|---|---|
| Mean [%] | SD [%] | n | No. of analysed transformants | Mean [%] | SD [%] | n | No. of analysed transformants | |
| alp1 | 93.9 | 9.4 | 7 | 108 | 80 | 7.1 | 4 | 78 |
| pks4.2 | 50 | 34.3 | 13 | 228 | 100 | 0 | 10 | 158 |
| snc1 | 57.2 | 17.7 | 11 | 185 | 40 | 10.4 | 6 | 118 |
| ptf1 | 7 | 6.6 | 3 | 28 | 5.5 | 7.8 | 2 | 38 |
SD standard deviation, n number of transformations performed
Diagnostic PCR was done for alp1 and ptf1 loci as described in “Methods” section. For snc1, fluorescence microscopy and for pks4.2, the colour of conidia was used to verify successful integration of the donor DNA. The donor DNA for pks4.2 was carrying the amdS selection marker