Fig. 6.

Respirasome amounts were decreased in HF mice and not restored by linoleic acid, whereas amounts of complex III₂/IV and complex II were partially restored by linoleic acid supplementation. a. Top: Amounts of supercomplexes (respirasome and complex III₂/IV) analyzed by BN-PAGE in mice fed a normal diet (n = 4), and HF mice fed a normal diet (n = 4) and linoleic acid-rich diet (n = 4). Total protein levels shown by CBB staining (bottom) were used as internal controls. The sizes of the molecular weight markers are indicated on the left in kDa. b. Amounts of complex II analyzed by BN-PAGE in mice fed a normal diet (n = 4), and HF mice fed a normal diet (n = 4) and a linoleic acid-rich diet (n = 4). Total protein levels shown by CBB staining were used as internal controls. The sizes of the molecular weight markers are indicated on the left in kDa. Data are shown as the mean ± SE. *p < 0.05 vs mice fed a normal diet. †p < 0.05 vs. HF mice fed a normal diet