Fig. 4.

The JNK/IRS1 pathway influenced by PYCR1 interference. a The heatmap of different expressed genes between shCtrl group and shPYCR1 group. Red represents up-regulated genes, and green represents down-regulated genes. Upper tree structure is listed according to the sample characteristics, and left tree structure is listed according to the gene characteristics. There is a higher similarity between the adjacent samples or genes. b The significantly altered canonical pathway between shCtrl group and shPYCR1 group. The result showed that SAPK/JNK was the most significantly altered signaling pathway. c The mRNA and protein expressions of c-Jun and IRS1 were determined by qRT-PCR and western blot. Both c-Jun and IRS1 were significantly down-regulated by PYCR1 interference in mRNA and protein levels (P < 0.001). GAPDH was as the internal standard. N = 3. Values were expressed as mean ± standard deviation