Fig. 3.

Notch-mediated proliferative effect requires functional PI3K/Akt and Wnt/β-catenin signaling. a, b Downregulation of Notch2/Hey2/Hes1 expression by siRNA led to a marked arrest in cell cycle progression, as characterized by an accumulation of NP cells in G0/G1 cells. c–f JAG2 treatment increased the expression of PI3K/Akt and Wnt/β-catenin signaling, which was inhibited by Notch2/Heys2/Hes1 siRNA. g, h The increases in cyclin D1 and CDK6 mRNA levels after JAG2 treatment were abrogated by treatment with LY294002 and XAV-939 alone or in combination. i–k Western blot and densitometric analyses showed similar results. P values were computed vs. non-stimulated controls* or JAG2-stimulated controls^#; *^,#P < 0.05