Supplementary Fig. 1.

Effect of cTnIAAb treatment on the expression of hypertrophic genes in vitro. Neonatal rat cardiomyocytes were treated for 48h with cTnIAAb (100 μg/L), cTnTmAb (100 μg/L), mouse IgG (100μg/L) and AngII (1μM, Sigma), respectively. AngII was used as positive control. Thereafter, total RNA was isolated using TRIzol reagent, and the hypertrophic markers, Nppa, Nppb and myh7/myh6 were quantified using RT-PCR and normalized to β-actin housekeeping gene. *p<0.05 compared to control (t-test, n=3).