cTnTmAb and mouse-IgG did not induce apoptosis in primary cultured cardiomyocytes. A. Treatment with mouse-IgG at different concentrations (0, 25μg/ml, 50μg/ml, 100μg/ml) or 50μg/ml mouse-IgG for 48h did not induce myocardial apoptosis as revealed by FACS. B. Treatment with cTnTmAb at different concentrations (0, 25μg/ml, 50μg/ml, 100μg/ml) or 50μg/ml cTnTmAb for 48h did not induce myocardial apoptosis as revealed by FACS. C. Treatment with mouse-IgG at different concentrations (0, 25μg/ml, 50μg/ml, 100μg/ml) or 50μg/ml mouse-IgG for 48h did not alter the expression of Bax, Bcl2 and cleaved Caspase3 as revealed by immunoblot analysis. β-actin served as a loading control. D. Treatment with cTnTmAb at different concentrations (0, 25μg/ml, 50μg/ml, 100μg/ml) or 50μg/ml cTnTmAb for 48h did not alter the expression of Bax, Bcl2 and cleaved Caspase3 as revealed by immunoblot analysis. β-actin served as a loading control.