Fig. 2.

CD6-targeting increases Th1 polarization while inhibiting Treg differentiation. OVA-specific TCR-transgenic OT-II.Rag^−/− CD4 T cells were cultured for 4 days in a 2:1 ratio with bone marrow derived dendritic cells (BMDC) in Th1 and Treg polarizing conditions. (a, b) Representative flow cytometry dot plots and scatter plots showing the percentage of CD25⁺Foxp3⁺ T cells within CD4⁺TCRβ⁺ T cells at the end of Treg polarizing cultures with different doses of anti-CD6 (10F12) or 100 μg/ml isotype control (IC). (c) Survival of CD4 T cells at the end of culture. (d) Number of CD4 T cells recovered at the end of the culture. (e) Representative histograms showing CTV dilution of T cells following culture and bar graph displaying the frequency of cells within gates representing low, intermediate and high proliferation as displayed in the histograms. (f, g) Representative flow cytometry dot plots and scatter plots showing the percentage of CD25⁺IFNγ ⁺ T cells within CD4⁺TCRβ⁺ T cells in Th1-polarizing cultures. (h) Viability of CD4 T cells under Th1 polarizing conditions. (i) Number of CD4 cells recovered at the end of culture. (j) T cell proliferation under Th1 polarizing conditions. (k) Representative dot plots and scatter plots showing the percentage of T cells producing IL-17 (top) or IL-13 (bottom) following culture under, respectively, Th17 and Th2 polarizing conditions as well as their viability (right). Statistical tests: Kruskal-Wallis and Mann-Whitney. Data are representative of three independent experiments, each with n = 3. *p < .05 **p < .01.