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. 2019 Aug 10;47:195–207. doi: 10.1016/j.ebiom.2019.07.068

Fig. 1.

Fig. 1

METTL3 and ALKBH5 affected cell adhesion by regulating ITGA6 expression in BC cells.

a, b Depletion of METTL3 (KO-METTL3) decreased the adhesion of T24 (a) or UM-UC-3 (b) cells. c Overexpression of METTL3 (OE-METTL3) enhanced the adhesion of SV-HUC-1 cells. d Depletion of ALKBH5 (KO-ALKBH5) enhanced the adhesion of SV-HUC-1 cells. e, f Overexpression of ALKBH5 (OE-ALKBH5) enhanced the adhesion of T24 (e) or UM-UC-3 (f) cells. g, h Western blotting of 14 cell adhesion molecules in OE-METTL3 (g) or KO-ALKBH5 (h) SV-HUC-1 cells. The chart (right) shows the relative ITGA6 integrated density in the Western blotting results. I, j Western blotting of ITGA6 in KO-METTL3 T24 (i) or KO-METTL3 UM-UC-3 (j) cells. The chart (right) shows the relative ITGA6 integrated density in the Western blotting results. K, l Western blotting of ITGA6 in OE-ALKBH5 T24 (k) or OE-ALKBH5 UM-UC-3 (l) cells. The chart (right) shows the relative ITGA6 integrated density in the Western blotting results. m RT-qPCR analysis of ITGA6 mRNA expression in stable cell lines. All bar plot data are the means ± SDs or means ± SEMs of three independent biological replicates. *p < .05, ** p < .01, *** p < .001, (Student's t-test).