Fig. 1.

PS in obese mice induces hypophagia and body weight loss. (a) Simplified cartoon of experimental design. The experimental groups are represented from top to bottom as follow: Control (n = 10), mice fed with a standard diet (SD) for 18 weeks; Ob (n = 10) mice fed with a high fat diet (HFD) for 18 weeks; PS (n = 10) mice fed with a standard diet for 18 weeks and subjected to the resident-intruder paradigm from week 16th to week 18th of the protocol; Ob + PS (n = 10) mice fed a high fat diet for 18 weeks and subjected to the resident-intruder paradigm from week 16th to week 18th. At the end of the protocol the analysis indicated on the right panel was performed. (b) Time-dependent effects of standard diet (control, n = 10; PS, n = 10) or high fat diet (Ob, n = 10; Ob + Ps, n = 10) on murine body weight and (c) caloric (kcal) intake. (d) Number attacks and (e) latency to the first attack of mice exposed to the resident-intruder paradigm. One-way ANOVA was performed for each temporal point of the curves represented in the panels b and c (*p < 0·05, **p < 0·01, and ***p < 0·001 Vs Controls; ^#p < 0·05, ^### p < 0·001 Vs Ob; ^$p < 0·05, ^$$$p < 0·001 Vs PS). Two-tailed Student's t-test was performed for each temporal point of the curves represented in d and e (***p < 0·001). All measurements are shown as mean ± SEM.