Fig. 2.

DGP inhibits ZIKV infection of HT1080, VERO, and CHME3 cells. HT1080, VERO, and CHME3 cells were challenged with ZIKV-MR766 (A) or ZIKV-RVPs (B) at an MOI of ~1 in conjunction with increasing concentrations of DGP. At 48 h post-challenge, infection was determined by measuring the percentage of 4G2-positive and GFP-positive cells for ZIKV-MR766 and ZIKV-RVPs infections, respectively. (C) CHME3 cells were challenged with ZIKV PRVABC59, ZIKV iBeH819015, ZIKV IbH3065, and ZIKV DAK ArD-51,254 at an MOI of 1, 0.2, 0.2 and 0.5, respectively. 48 h post-challenge, infection was determined by measuring the percentage of 4G2-positive cells. The IC₅₀ and selectivity index (SI) values are shown for each of the dose-dependent curve. The SI was calculated by the ratio of CC₅₀ to IC₅₀. Experiments were repeated at least three times and results of a representative experiment are shown. Bars represent the Mean ± SD. P < .05 (*), P < .01 (**), P < .001 (***), or not significant (ns), using two-tailed Student's t-test are shown.