Fig. 4.

DGP blocks infectivity of other Flaviviruses. DENV1, JEV, TBEV, WNV, and EBV reporter viral particles expressing GFP were used to challenge VERO cells in the presence of increasing concentrations of DGP. At 48 h post-challenge, infection was determined by measuring the percentage of GFP-positive cells using a flow cytometer. Infection values were normalized to the values of infection without DGP. Experiments were repeated at least three times and results of a representative experiment are shown. Bars represent the Mean ± SD. P < .05 (*), P < .01 (**), P < .001 (***), or not significant (ns), using two-tailed Student's t-test are shown.