Fig. S2.

Cytotoxic concentration 50 (CC₅₀). The CC₅₀ is the concentration of the drug that kills 50% of the cells in culture. Cell viability was determined by measuring the reduction in the levels of the tetrazolium dye MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide], which is converted to its insoluble form formazan. We treated 4 × 10³ cells/well in a 96-well plate with the indicated concentrations of DGP, diphyllin, or 6-Deoxy-Glucose for 48 h at 37 °C. After the incubation period, 10 μl MTT solution (5 mg/mL) was added to each well for an additional 4 h at 37 °C. Finally, the medium was removed and dimethyl sulfoxide was added (200 μl/well) according to the manufacturer's instructions. The optical density was measured at 570 nm using a microplate reader. Experiments were performed in triplicates and standard deviations are shown. Mock-treated cells represent 100% viability.