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. 2019 Aug 13;47:208–220. doi: 10.1016/j.ebiom.2019.08.012

Fig. 3.

Fig. 3

TAGLN promotes the migration and invasion of BLCA cells in vitro and in vivo. a. RT-qPCR and western blot analyses of TAGLN in the normal uroepithelium cell line (SV-HUC-1) and BLCA cells (SW780, 5637 and T24). b. RT-qPCR and western blot analyses of BLCA cells infected with a lentivirus-mediated TAGLN-overexpressing vector or TAGLN shRNAs. c. Clone formation assay. Representative images of plates seeded with BLCA cells and quantification of colony formation after 10 day. d. Wound-induced migration assay. Representative images of wound-induced cell migration by the T24-shTAGLN, 5637-TAGLN and control cells. Scale bars, 100 μm. And quantification of migration by the described cells. e. Transwell migration and invasion assay. Representative images of transwell migration and invasion assay of TAGLN-silenced cells and TAGLN-overexpressed cells. Scale bars, 100 μm. And quantification of transwell migration and invasion by the described cells. f-g. Tail vein-injected T24-luc metastasis model. Representative IVIS images of mice injected TAGLN-silenced or control cells and analysis of tumour luminescence representing lung metastasis measured on day 42. Five mice in each group (f). Lung metastasis was confirmed by H&E and IHC-TAGLN staining. Scale bars, 250 μm and 100 μm. And quantification of the metastatic lesion(g). Error bars represent SD of the mean. *, P < .05; **, P < .01.