Fig. 4.

TAGLN promotes invadopodia formation and EMT. a. Representative anti-TAGLN immunofluorescence images of TAGLN-silenced cells and TAGLN-overexpressed cells. Cells were counterstained with DAPI; combined images were indicated. Scale bar, 10 μm. b. Representative invadopodia immunofluorescence images in BLCA cells. TAGLN-silenced cells, TAGLN-overexpressed cells and control cells stained for nuclei (blue), F-actin (red), and. CTTN (green). Quantification of the percentage of cells with invadopodia. N = 50 cells/sample. Scale bars, 10 μm in T24 and SW780(a); 5 μm in 5637(b). c-d. Western blot and RT-qPCR analyses of EMT marker and transcription factors after TAGLN depletion in T24 (c) and TAGLN overexpression in SW780 and 5637 (d). e. Representative IHC staining images of TAGLN and Slug in lung metastatic of tail vein-injected T24-Luc metastasis model. Scale bars, 50 μm. Error bars represent SD of the mean. *, P < .05; **, P < .01. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)