Supplementary Fig. S2.

Function and mechanism studies of in BLCA cell lines. a. Cell viability assay showed cell count in replicate cultures cells for five days. b. Cell cycle assay. Representative images of cells stained with PI measured by flow cytometer and percentage distribution of cells in different phases of the cell cycle. c. TAGLN and CTTN expression level of 5637, SW780 and T24 in CCLE. d. Western blot analyses of CTTN and TAGLN of 5637, SW780 and T24. e. Representative merged immunofluorescence images of invadopodia in T24 and 5637. Scale bars, 50 μm and 25 μm in T24; 50 μm and 10 μm in 5637. And quantification of the percentage of BLCA cells with invadopodia. N = 50 cells/sample. Error bars represent SD of the mean. *, P < .05. d.