Fig. 1.

CD4i epitope-specific ADCC in MTCT cohort.

Results of the competition RFADCC assay performed on 142 plasma samples from breastfeeding Kenyan mother-infant pairs. a-c: Percent CD4i epitope-specific (referred to as CD4i antibody-like) ADCC activity (100% - relative ADCC) was calculated for each plasma sample in the presence of A32-LALA (a), C11-LALA (b), and 17b-LALA (c). Negative values were treated as zeros (grey points). d-f: Percent LALA-mediated ADCC enhancement (relative ADCC - 100%) was calculated for each plasma sample in the presence of A32-LALA (d), C11-LALA (e), and 17b-LALA (f) Negative values were treated as zeros (orange points). Results are averaged from two biological replicates. Error bars represent median + interquartile range. Data from individual biological replicates from seven maternal samples and six infant samples that were below the limit of detection were excluded from the analysis as described in the Materials and Methods.