Fig. 5.

Inhibition of glycolysis impairs miR-125a-induced pro-angiogenic phenotype.

Bar graph quantifying the inhibition of anti-miR-125a induced cell migration (A), invasion (B) and network formation (C) in response to 3PO (n = 3). Data are represented as mean ± SEM, *p < .05, significantly different to control. (D) Representative fluorescent images demonstrating vascular hyper-branching (red arrows) in miR-125a1^−/− embryos in the presence of DMSO (n = 5). Treatment of miR-125a1^−/− embryos with 3PO largely normalised the hyper-branching phenotype (n = 5). (E) Incidence of phenotype severity showing the no, mild, moderate and severe hyper-vascular branching in miR-125a1^−/− in the presence of 3PO or DMSO vehicle control. Data are presented as phenotype frequencies. Statistical significance was analysed by the chi-square test. ***p < .001, significantly different to control. See also Fig. S7. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)