Fig. 5.

C1-inhibitor reduced the release of C3- and C9-positive endothelial microvesicles after perfusion of C1-inhibitor-depleted plasma over primary glomerular endothelial cells.

C1-inhibitor-depleted plasma was perfused over primary glomerular endothelial cells (PGECs) and shed C3- and C9-positive endothelial microvesicles (EMVs) were detected by flow cytometry. EMVs in pre-perfusion samples have been deducted from perfused samples. A) C1-inhibitor-depleted plasma perfused over PGECs released EMVs positive for C3 that were higher than control plasma (median EMVs in control plasma was 5·9 × 10⁷/mL). Addition of C1-inhibitor to the C1-inhibitor-depleted plasma significantly reduced the C3-positive EMVs. B) C1-inhibitor-depleted plasma perfused over PGECs released EMVs positive for C9 that were higher than control plasma (median in control plasma 5·1 × 10⁶/mL). Addition of C1-inhibitor to the C1-inhibitor-depleted plasma significantly reduced the C9-positive EMVs. **: P < 0·01, *: P < 0·05. C1INH: C1-inhibitor. Dpl: depleted. The bar represents the median. Samples were analysed using a CyFlow Cube 8 flow cytometer.